Source:Mouse myeloma cell line, NS0-derived, Ser293-Arg408
N-terminal Sequence Analysis:Ser293
Purity:>95%, by SDS-PAGE with silver staining.
Predicted Molecular Mass:13 kDa (monomer)
SDS-PAGE:22-25 kDa, reducing conditions
37-41 kDa, non-reducing conditions
Activity:Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.
The ED50 for this effect is typically 2.5-15 ng/mL.