Products

Recombinant Human BMP‑4

DESCRIPTION
CATALOG #
SIZEPRICEQTY 
Recombinant Human BMP-4
314-BP-01M 1 mg Please Inquire
Recombinant Human BMP-4
314-BP-010 10 µg $365
Recombinant Human BMP-4
314-BP-050 50 µg $959
Recombinant Human BMP-4, CF
314-BP-010/CF 10 µg $365
Recombinant Human BMP-4, CF
314-BP-050/CF 50 µg $959

Source:Mouse myeloma cell line, NS0-derived, Ser293-Arg408
Accession #:Q53XC5
N-terminal Sequence Analysis:Ser293
Structure:Disulfide-linked homodimer
Purity:>95%, by SDS-PAGE with silver staining.
Predicted Molecular Mass:13 kDa (monomer)
SDS-PAGE:22-25 kDa, reducing conditions
37-41 kDa, non-reducing conditions
Activity:Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.
The ED50 for this effect is typically 2.5-15 ng/mL.
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

Which formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

314-BP 314-BP/CF

Formulation: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details.

Formulation: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details.

Reconstitution: Reconstitute at 50-200 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Reconstitution: Reconstitute at 50-200 µg/mL in sterile 4 mM HCl.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.