Products

Human CXCL8/IL-8 Quantikine ELISA Kit

DESCRIPTION
CATALOG #
SIZEPRICEQTY 
Human CXCL8/IL-8 Quantikine ELISA Kit
D8000C 1 Kit $505
Human CXCL8/IL-8 Quantikine PharmPak (50 Plates)
PD8000C 1 Pack Please Inquire
Human CXCL8/IL-8 Quantikine SixPak (6 Plates)
S8000C 1 Pack $2,419

Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:3.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL)
Sensitivity:7.5 pg/mL
Assay Range:31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
Specificity:Natural and recombinant human IL-8
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:No significant interference observed with available related molecules.
Product Summary:

The Quantikine Human IL-8 Immunoassay is a 3.5 hour solid phase ELISA designed to measure human IL-8 in cell culture supernates, serum, and plasma. It is based on antibodies raised against the 72 aa variant of human IL-8 derived from E. coli. It is calibrated with the same recombinant factor. This immunoassay accurately quantitates recombinant human IL-8. Measurement of natural human IL-8 or the 77 aa variant of human IL-8 gave results parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.


Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)hree samples of known concentration were tested in separate assays to assess inter-assay precision.

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 168 526 1,093 196 581 1,160
Standard Deviation 9.4 28.6 70.7 14.5 56.6 70.2
CV% 5.6 5.4 6.5 7.4 9.7 6.1

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 115 386 802 132 410 817
Standard Deviation 5.3 17 37.9 10.7 28 42.4
CV% 4.6 4.4 4.7 8.1 6.8 5.2


Recovery:

The recovery of IL-8 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 85-114
Serum (n=5) 98 88-106
EDTA Plasma (n=5) 103 97-111
Heparin Plasma (n=5) 102 92-107
Citrate Plasma (n=5) 105 95-114


Linearity:

To assess the linearity of the assay, samples were spiked with high concentrations of IL-8 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.