Human Angiogenin Quantikine ELISA Kit

Human Angiogenin Quantikine ELISA Kit
DAN00 1 Kit $479
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Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:2.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (200 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity:6 pg/mL
Assay Range:78.1 - 5,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
Specificity:Natural and recombinant human Angiogenin
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference:No significant interference observed with available related molecules.
Product Summary:

The Quantikine Human Angiogenin Immunoassay is a 2.5 hour solid phase ELISA designed to measure Angiogenin in cell culture supernates, serum, and plasma. It contains recombinant human Angiogenin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate both natural and recombinant human Angiogenin.


Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 356 717 1,739 664 1,911 3,671
Standard Deviation 10 24 50 47 166 309
CV% 2.8 3.3 2.9 7.1 8.7 8.4


The recovery of Angiogenin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=10) 103 84-126
Heparin Plasma (n=10) 102 80-137
Citrate Plasma (n=10) 102 84-126
EDTA Plasma (n=10) 101 79-121
Cell Culture Media (n=4) 107 97-117


The following samples were diluted with Calibrator Diluent and assayed to assess linearity of the assay.