Products

Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

DESCRIPTION
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Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
DFL00 1 Kit $475

Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:4.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity:8.05 pg/mL
Assay Range:15.6 - 1,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
Specificity:Natural and recombinant human Fas Ligand
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:No significant interference observed with available related molecules.
Product Summary:

The Quantikine Human Fas Ligand Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fas Ligand in cell culture supernates, cell lysates, serum, and plasma. It contains CHO cell-expressed recombinant human Fas Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fas Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fas Ligand.


Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 103 261 624 98.3 261 625
Standard Deviation 4.8 10.7 34 8.3 22.9 40
CV% 4.7 4.1 5.4 8.4 8.8 6.4


Recovery:

The recovery of Fas Ligand spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=4) 101 92-111
Heparin Plasma (n=4) 98 89-115
EDTA Plasma (n=4) 99 90-114
Cell Culture Media (n=4) 101 94-109


Linearity:

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Fas Ligand were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.