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Human Follistatin Quantikine ELISA Kit

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Human Follistatin Quantikine ELISA Kit
DFN00 1 Kit $519

Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:5.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (10 µL), Serum (100 µL), EDTA Plasma (100 µL), Follicular Fluid (10 µL)
Sensitivity:83 pg/mL
Assay Range:250 - 16,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma, Follicular Fluid)
Specificity:Natural and recombinant human Follistatin
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:Interference observed with 1 or more available related molecules.
Product Summary:

The Quantikine Human Follistatin Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human Follistatin (FS288, FS300, and FS315) in follicular fluid, cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human Follistatin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human Follistatin. Results obtained using natural Follistatin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural Follistatin.


Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1,850 5,363 10,734 1,645 4,850 10,256
Standard Deviation 59 136 181 139 312 584
CV% 3.2 2.5 1.7 8.4 6.4 5.7

Serum, EDTA Plasma, Follicular Fluid
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1,996 6,047 11,999 1,888 5,604 11,626
Standard Deviation 53 144 239 173 399 918
CV% 2.7 2.4 2 9.2 7.1 7.9


Recovery:

The recovery of Follistatin spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=5) 95 87-104
EDTA Plasma (n=5) 96 87-102
Cell Culture Media (n=4) 97 91-107


Linearity:

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Follistatin were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.