Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
DSA00 1 Kit $509
Human CXCL12/SDF-1 alpha Quantikine PharmPak (50 Plates)
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Human CXCL12/SDF-1 alpha Quantikine SixPak (6 Plates)
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Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:4.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (10 µL), EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)
Sensitivity:47 pg/mL
Assay Range:156 - 10,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma)
Specificity:Natural and recombinant SDF-1 alpha
Cross-reactivity:Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference:No significant interference observed with available related molecules.
Product Summary:

The Quantikine Human SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma. It contains E. coli-expressed recombinant human SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SDF-1 alpha.


Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 491 1,700 3,420 544 1,600 3,150
Standard Deviation 19 58 115 73 150 257
CV% 3.9 3.4 3.4 13.4 9.4 8.2


The recovery of SDF-1 alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Platelet-poor EDTA Plasma (n=5) 97 90-107
Platelet-poor Heparin Plasma (n=5) 97 90-104
Cell Culture Media (n=4) 101 95-108


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.