Products

Human VEGF Quantikine ELISA Kit

DESCRIPTION
CATALOG #
SIZEPRICEQTY 
Human VEGF Quantikine ELISA Kit
DVE00 1 Kit $519
Human VEGF Quantikine PharmPak (50 Plates)
PDVE00 1 Pack Please Inquire
Human VEGF Quantikine SixPak (6 Plates)
SVE00 1 Pack $2,491

Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:4.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (200 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
Sensitivity:9 pg/mL
Assay Range:15.6 - 1,000 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)
Specificity:Natural and recombinant human VEGF
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference:Interference observed with 1 or more available related molecules.
Product Summary:

The Quantikine Human VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF165 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VEGF165 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human VEGF and recombinant human VEGF121 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human VEGF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF.


Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 53.7 235 910 64.5 250 1,003
Standard Deviation 3.6 10.6 46.2 5.7 17.4 61.7
CV% 6.7 4.5 5.1 8.8 7 6.2

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 29.1 123 531 32.8 128 495
Standard Deviation 1.9 5 18.4 2.8 6.4 33
CV% 6.5 4.1 3.5 8.5 5 6.7


Recovery:

The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=5) 102 92-115
Heparin Plasma (n=5) 93 82-102
Citrate Plasma (n=5) 100 88-113
EDTA Plasma (n=5) 97 82-113
Cell Culture Media (n=5) 102 95-111


Linearity:

To assess the linearity of the assay, samples spiked with high concentrations of VEGF were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.