Products

Human TNF-alpha DuoSet

DESCRIPTION
CATALOG #
SIZEPRICEQTY 
Human TNF-alpha DuoSet, 15 Plate
DY210 1 Kit $649
Human TNF-alpha DuoSet, 5 Plate
DY210-05 1 Kit $249
Human TNF-alpha DuoSet BulkGet Quote Bulk/Custom

Assay Type:ELISA Development Kit
Assay Length:4 hours 40 minutes (after plate preparation)
Sample Type & Volume Required:100 µL
Sufficient Materials:For fifteen 96-well plates*
Range:15.6 - 1,000 pg/mL
Specificity:Please see the product datasheet
PRODUCT DETAIL

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant Human TNF-alpha. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.


PRODUCT FEATURES
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

KIT CONTENTS
  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

OTHER REAGENTS REQUIRED FOR DUOSET DEVELOPMENT KITS
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product datasheet.