Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:6.5 hours
Sample Type & Volume Required Per Well:Serum (150 µL), EDTA Plasma (150 µL), Heparin Plasma (150 µL)
Assay Range:0.125 - 8 pg/mL (Serum, Heparin Plasma, EDTA Plasma)
Specificity:Natural and recombinant human IL-1 beta
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:No significant interference observed with available related molecules.
The Quantikine HS Human IL-1 beta Immunoassay is a 6.5 hour solid phase ELISA designed to measure human IL-1 beta levels in serum and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor and has been shown to accurately quantitate recombinant human IL-1 beta. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in serum and plasma.
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of IL-1 beta was determined by spiking to levels throughout the range of the assay in various matrices.
To assess the linearity of the assay, samples were spiked with high concentrations of IL-1 beta and serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.