Products

Mouse/Rat FGF-21 Quantikine ELISA Kit

DESCRIPTION
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Mouse/Rat FGF-21 Quantikine ELISA Kit
MF2100 1 Kit $479

Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:4.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity:13.4 pg/mL
Assay Range:31.3 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
Specificity:Natural and recombinant mouse and rat FGF-21
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:No significant interference observed with available related molecules.
Product Summary:

The Quantikine Mouse/Rat FGF-21 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FGF-21 in mouse or rat cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse FGF-21 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse FGF-21. Results obtained using natural mouse or rat FGF-21 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF-21.


Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 84.4 226 754 94.5 231 745
Standard Deviation 5.24 9.6 19.7 6.61 10.8 31
CV% 6.2 4.2 2.6 7 4.7 4.2

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 118 317 1,032 120 302 977
Standard Deviation 7.35 13.5 25.2 8.91 18 65.9
CV% 6.2 4.3 2.4 7.4 6 6.7


Recovery:

The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Samples (n=6) 96 88-101
Rat Cell Culture Samples (n=2) 91 87-95
Mouse Serum (n=4) 95 83-103
Rat Serum (n=4) 100 93-108
Mouse EDTA Plasma (n=4) 97 86-104
Rat EDTA Plasma (n=4) 101 93-119
Rat Heparin Plasma (n=4) 97 94-104
Mouse Heparin Plasma (n=4) 93 83-101


Linearity:

To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 in each matrix were diluted with the appropriate Calibrator Diluent and assayed.