Products

Mouse VEGF Quantikine ELISA Kit

DESCRIPTION
CATALOG #
SIZEPRICEQTY 
Mouse VEGF Quantikine ELISA Kit, 2 Plate
MMV00 1 Kit $629
Mouse VEGF Quantikine PharmPak (50 Plates)
PMMV00 1 Pack Please Inquire
Mouse VEGF Quantikine SixPak (6 Plates)
SMMV00 1 Pack $1,509

Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:4.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity:3 pg/mL
Assay Range:7.8 - 500 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma, Tissue Homogenates)
Specificity:This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:Interference observed with 1 or more available related molecules.
Product Summary:

The Quantikine Mouse VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse VEGF in cell culture supernates, tissue homogenates, mouse serum, and plasma. It contains Sf 21-expressed mouse VEGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse VEGF accurately. Results obtained using natural mouse VEGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse VEGF.


Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 37.7 144 277 38.3 138 291
Standard Deviation 3.1 6.2 12.9 3.2 7.8 18.5
CV% 8.2 4.3 4.7 8.4 5.7 6.4


Recovery:

The recovery of mouse VEGF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=9) 101 83-115
Heparin Plasma (n=4) 89 87-94
Cell Culture Supernates (n=9) 103 95-114
EDTA Plasma (n=5) 91 82-103
Tissue Homogenates (n=3) 91 76-107


Linearity:

To assess the linearity of the assay, samples containing and/or spiked with various concentrations of mouse VEGF in each matrix were diluted with Calibrator Diluent and then assayed.