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Mouse Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

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Mouse Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
MSHH00 1 Kit $479
Mouse Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit BulkGet Quote Bulk/Custom

Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:4.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (50 µL), Serum (50 µL), Heparin Plasma (50 µL)
Sensitivity:2.37 pg/mL
Assay Range:7.81 - 500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates)
Specificity:Natural and recombinant mouse Shh-N. This kit also recognizes low levels of Shh-N in rat serum.
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:No significant interference observed with available related molecules.
Product Summary:

The Quantikine Mouse Sonic Hedgehog N-Terminus Immunoassay is a 4.5 hour solid phase ELISA designed to measure Shh-N in cell culture supernates, serum, and plasma. It contains recombinant mouse Shh-N and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Shh-N showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Shh-N.


Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 44 44
Mean 22.7 54.6 212 21.9 50.4 188.7
Standard Deviation 1.08 1.31 10.6 2.28 2.96 9.21
CV% 4.8 2.4 5 10.4 5.9 4.9


Recovery:

The recovery of mouse Shh-N spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=4) 98 86-109
Heparin Plasma (n=4) 97 86-108
Cell Culture Samples (n=6) 101 96-109


Linearity:

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse Shh-N were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.