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Rat Total MMP-9 Quantikine ELISA Kit

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Rat Total MMP-9 Quantikine ELISA Kit
RMP900 1 Kit $479
Rat Total MMP-9 Quantikine ELISA Kit BulkGet Quote Bulk/Custom

Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:4.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor Heparin Plasma (10 µL)
Sensitivity:0.028 ng/mL
Assay Range:0.156 - 10 ng/mL
Specificity:Natural and recombinant rat MMP-9 (Pro-, active, and TIMP-complexed)
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:No significant interference observed with available related molecules.
Product Summary:

The Quantikine Rat Total MMP-9 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total rat MMP-9 (Pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat MMP-9 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obtained using natural rat MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat MMP-9.


Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, Platelet-poor Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.39 1.22 5.27 0.51 1.18 3.55
Standard Deviation 0.027 0.068 0.238 0.035 0.046 0.153
CV% 6.9 5.6 4.5 6.9 3.9 4.3


Recovery:

The recovery of rat MMP-9 spiked into various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 94 87-107
Serum (n=4) 97 90-115
Platelet-poor Heparin Plasma (n=4) 92 80-103


Linearity:

To assess the linearity of the assay, samples containing high concentrations of rat MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.