Researchers employing stem or progenitor cells in their studies currently
use a wide variety of media products to support their cultures. Unfortunately,
these media are generally developed or validated for cells other than stem
cells, thus making them less desirable than cell type-specific media.
To address this need, R&D Systems offers two new specialty media products
for stem/progenitor cell culture. All the components included within these
defined, basal, serum-free formulations have been selected, optimized, and
validated for primary stem cell culture. StemXVivo Serum-free HSC Base Media
is formulated for the maintenance and expansion of purified human CD34+ hematopoietic
stem cells. StemXVivo Serum-free NSC Base Media is formulated for expansion
and differentiation of human and rodent neural stem and progenitor cells.
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| Figure 1. Expansion of rat cortical stem cells (Catalog # NSC001). For monolayer culture, cells were expanded using R&D Systems Fibronectin-coated plates (Catalog # 1030-FN) and StemXVivo Serum-Free NSC Base Media (Catalog # CCM002) supplemented with recombinant human FGF-basic (Catalog # 233-FB; 20 ng/mL ). For neurosphere culture, cells were expanded using the StemXVivo Serum-Free NSC Base Media supplemented with recombinant human FGF-basic and EGF (Catalog # 236-EG; 20 ng/mL). |
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| Figure 2. Expansion of total nucleated cells (TNCs; A) and CD34+ cells (B) from G-CSF mobilized human CD34+ cells. Cells were cultured in R&D Systems StemXVivo Serum-Free HSC Base Media (Catalog # CCM001) supplemented with 100 ng/mL each R&D Systems recombinant human Flt-3 Ligand (Catalog # 308-FKN), SCF (Catalog # 255-SC), and Tpo (Catalog # 288-TP). On days 10 and 14, cells were harvested and assayed for TNC and CD34+ cell expansion. Viable TNCs were counted using a hemocytometer and the CD34
expression was analyzed by flow cytometry. |
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