beta-Catenin is a downstream component of canonical Wnt signaling pathways. In
unstimulated cells, beta-catenin is phosphorylated by the kinase GSK-3, and
subsequently targeted for proteasomal degradation. Wnt binding to its receptors
Frizzled and LRP-5/6, supÂpresses beta-catenin phosphorylation. beta-Catenin
then accumulates and binds to LEF/TCF transcription factors resulting in the
activation of Wnt target genes. The canonical Wnt pathway plays a role in cellular
proliferation, differentiation, and motility, and has been implicated in tumor
formation. Therefore, it is important to have quality research reagents for
the study of beta-catenin and its activities. For an updated listing of other
Wnt-related research reagents including antibodies, proteins, and ELISAs designed
to study ligands, receptors, inhibitors, and intracellular signaling mediators,
go to
www.RnDSystems.com/go/Wnt
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| Figure 1. Lysates prepared from human MCF-7, HeLa, A431, SK-MEL-28, and
DLD-2C2 cells were quantified with R&D Systems Human Total b-Catenin
DuoSet® IC ELISA (Catalog # DYC1329). The same cell lysates were also
immunoblotted (inset) with R&D Systems anti-b-catenin monoclonal antibody (Catalog
# MAB1329). The DuoSet IC ELISA results correlate well with the total amount
of beta-catenin detected by Western blot. |
Figure 2. beta-Catenin was detected in sections of paraffin embedded human
kidney cancer tissues using R&D Systems anti-human/mouse/rat beta-catenin
monoclonal
antibody (Catalog # MAB1329). Tissues were stained using R&D Systems
anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown)
and counterstained with hematoxylin (blue). The inset shows control staining
without primary antibody. |
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