R&D Systems ExactaChIP Chromatin IP Kits are designed to provide a fast, simple method for the identification of genomic DNA target sequences bound by a specific transcription factor. Protein-DNA complexes are fixed by formaldehyde crosslinking, the chromatin is sheared, and the complex is immunoprecipitated using an antibody specific for the target protein. Protein-bound DNA fragments are purified and detected by standard PCR.
- Antibodies have been validated for chromatin immunoprecipitation
- Fast - results can be obtained in 4 - 5 hours
- A negative control antibody is included with each kit
- Consistent results from one experiment to the next
- Detailed, easy to follow protocols and troubleshooting guide are provided
- Positive control primer set included with each kit provides confidence in the experimental results obtained