R&D Systems is pleased to offer a new screening tool to identify modulators of neural progenitor cell proliferation and differentiation. The 96-well format provides an easy method to screen for toxins, drugs, growth factors, and other substances that may affect neural stem cells. The Neural Toxicity Assay Kit (Catalog # SC014) can be used to determine the effects on both proliferation and differentiation; either adverse effects as with a toxin or increased rates of proliferation and differentiation resulting from a stimulatory agent.
Kit Components
- Supplements for maintenance and differentiation
- Fibronectin for plate coating
- Resazurin to monitor proliferation
- beta-III tubulin ELISA to monitor differentiation
- Detailed protocols and sample data
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Effect of Ethanol (EtOH) on neuronal differentiation of neural progenitor cells. EtOH has been shown to reduce levels of neuronal differentiation of neural progenitor cells. Rat cortical stem cells (Catalog # NSC001) were differentiated in the presence of the indicated amounts of EtOH. Levels of neuronal differentiation were determined using the Neural Toxicity Assay Kit (Catalog # SC014) as assessed by beta-III Tubulin levels. Cells were stained with DAPI as a relative measure of total cell number. Constant levels of DAPI staining indicate that decreases in beta-III Tubulin levels are the result of reduced differentiation rather than cell death. |