Technical Information

Technical Information

New Tools: NorthernLights™ New Fluorescent Secondary Antibodies

With more than 6000 primary antibodies available, the addition of the NorthernLights line of fluorescent secondary antibodies makes R&D Systems your total source for immunofluorescence reagents.

NorthernLights Antibody/Label Catalog # Abs/Em Maxima Laser (Ex) Comparable Fluorochromes
NL-493 anti-Rabbit IgG
NL-493 anti-Mouse IgG
NL-493 anti-Goat IgG
NL-493 Streptavidin
NL009
NL003
NL997
NL006
493/514 Argon (488) FITC (492/520)
Cy™2 (489/506)
Alexa Fluor® 488 (494/519)
NL-557 anti-Rabbit IgG
NL-557 anti-Mouse IgG
NL-557 anti-Goat IgG
NL-557 Streptavidin
NL007
NL004
NL999
NL001
557/575 Krypton (568)
HeNe (543)
Phycoerythrin (565/575)
Rhodamine Red X (570/590)
Cy™3 (548/562)
NL-637 anti-Rabbit IgG
NL-637 anti-Mouse IgG
NL-637 anti-Goat IgG
NL-637 Streptavidin
NL005
NL008
NL002
NL998
637/658 HeNe (633) Allophycocyanin (645/660)
Alexa Fluor® 647 (650/668)
Cy™5 (650/670)

NorthernLights are bright and fade-resistant antibodies recognizing mouse, goat, and rabbit IgG. Like all R&D Systems antibodies, NorthernLights antibody conjugates are highly specific and deliver a high signal-to-noise ratio. Also available are streptavidin conjugates for labeling biotinylated cells and tissues. Their spectral properties are similar to several widely used fluorochromes, and are therefore suitable for use with common filter sets and/or lasers (Table). These secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy (Figure 1). NorthernLights secondary antibodies are stable when exposed to alcohols and xylene, as well as DPX mounting medium. They have been tested for their suitability in both immunochemistry and flow cytometry (Figure 2 and 3).

Figure 1 Figure 2
Figure 1. Rat cortical stem cell differentiation was monitored using multi-color immunocytochemistry. Neural progenitors were labeled with R&D Systems goat anti-rat nestin polyclonal antibody (Catalog # AF2736) and stained with R&D Systems donkey anti-goat NorthernLights-493 secondary antibody (Catalog # NL003; green). Differentiated neurons were labeled with R&D Systems neuron-specific mouse anti-beta-III tubulin monoclonal antibody (TuJ1; Catalog # MAB1195) and stained using R&D Systems donkey anti-mouse NorthernLights-557 secondary antibody (Catalog # NL007; red). Nuclei were stained with DAPI (blue). Figure 2. Glucagon is selectively expressed by some cells of the mouse pancreatic beta cell line beta-TC6. Glucagon was detected using R&D Systems anti-human/mouse monoclonal antibody and cells were stained with R&D Systems NorthernLights-557 secondary antibody (Catalog # NL007; red). The nuclei were counterstained with DAPI (blue).
Figure 3
Figure 3. Jurkat T cells were fixed, permeabilized, and incubated with R&D Systems anti-human Akt1 monoclonal antibody (Catalog # MAB17751). Cells were then stained with R&D Systems anti-mouse IgG NorthernLights-637 (Catalog # NL008; red histogram). Control stain­ing was done by incubating cells in R&D Systems IgG1 isotype control monoclonal antibody (Catalog # MAB002), followed by the staining with NorthernLights-637 (black histogram).

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