Axl was detected in immersion fixed paraffin-embedded sections of human stomach using Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to endothelial cells in the vasculature of the longitudinal muscle layer. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

This antibody specifically recognizes human Axl extracellular domain. Reactivity with Axl from other species has not been determined.

Axl phosphorylated at Y779 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Human/Mouse Phospho-Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.