Technical Information

IHC Images

IHC/ICC: PD-1

PD-1 in Mouse Thymus.
PD-1 in Mouse Thymus.

Programmed Death-1 (PD-1) was detected in perfusion fixed frozen sections of mouse thymus using Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) at 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

This antibody specifically recognizes mouse PD-1. In these formats, this antibody shows approximately 50% cross-reactivity with recombinant human PD-1.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.


PD‑1 in Human Lymph Node.
PD‑1 in Human Lymph Node.

Programmed Death-1 (PD‑1) was detected in immersion fixed paraffin-embedded sections of human lymph node using Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.