Transforming Growth Factor beta (TGF-beta) was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Human TGF-beta Monoclonal Antibody (Catalog # MAB240) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in prostate gland. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Mature TGF-beta1 is a highly conserved protein. This antibody detects TGF-beta1 from human, mouse, rat, and other species.

Transforming Growth Factor-beta (TGF-beta) was detected in immersion fixed PC-3 human prostate cancer cells using 10 µg/mL Mouse TGF-beta Monoclonal Antibody (Catalog # MAB1835) for 3 hours at room temperature. Cells were stained using R&D Systems NorthernLights™ 557 Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

This antibody recognizes bovine, mouse, and human TGF-beta 1 and TGF-beta 2. Recognition of TGF-beta 3 has been characterized in the chicken. As a result of greater than 97% TGF-beta homology between species, this antibody also may recognize the TGF-beta forms from a number of other species.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.