TNF Receptor-I in Human Peripheral Blood Lymphocytes.
Indirect immunofluorescent staining of TNF Receptor-I producing human peripheral blood lymphocytes using 5 µg/mL of R&D Systems goat anti-human TNF Receptor-I antibody (
Catalog # AF225) and counterstained (green). See
IHC Fluorescence Protocol 1.0.
This antibody has been selected for its ability to recognize human sTNF Receptor-I. Based on direct ELISA and Western blot results (non-reducing conditions), this antibody shows less than 10% cross-reactivity with recombinant mouse sTNF Receptor-I. This anti-human sTNF RI antibody can interact with the cell surface 55 kDa human TNF RI and exhibits TNF agonist activities on the human cell line A549. Additionally, this antibody also exhibits TNF agonist activities on the murine L-929 cell line, suggesting that it has cross species activity with the murine cell surface TNF RI. This antibody can be used for immunohistochemistry, Western blot and ELISA.
Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multicolor fluorescence microscopy.