Olig2 was detected in 7 day differentiated RCSC using 10 µg/mL goat anti-human Olig2 affinity-purified polyclonal antibody (Catalog # AF2418). Oligodendrocyte Marker O4 (O4) was detected in 7 day differentiated RCSC using 10 µg/mL mouse anti-human/mouse/rat/chicken O4 monoclonal antibody (Catalog # MAB1326). Cells were stained for Olig2 using R&D Systems NorthernLights™-637 donkey anti-goat IgG secondary antibody (red; Catalog # NL002), and stained for O4 using anti-mouse IgM secondary antibody (pseudo-stained green).

The Olig2 antibody was selected for its ability to recognize human Olig2, it also detects rat Olig2. This antibody can be used for immunocytochemistry, direct ELISA, and Western blot.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multicolor fluorescence microscopy.