Detection of sleeping beauty (SB) transposase (nuclear labeling) in paraffin-embedded liver tissue sections from SB transposase transgenic mouse using 5 µg/mL of R&D Systems rat anti-mouse SB transposase monoclonal antibody (Catalog # MAB2798). Note a lack of SB transposase immunoreactivity in tissues from a wild type mouse (inset). Before incubation with primary antibody tissues were subjected to heat-induced epitope retrieval using R&D Systems basic antigen retrieval solution (Catalog # CTS013). Staining was done using R&D Systems anti-mouse HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS002). See IHC Enzymatic Protocol 1.0. Tissues were provided by Dr. Lara Collier at the University of Minnesota.

Detection of sleeping beauty (SB) transposase (nuclear labeling) in paraffin-embedded intestine tissue sections from SB transposase transgenic mouse using 5 µg/mL of R&D Systems rat anti-mouse SB transposase monoclonal antibody (Catalog # MAB2798). Note a lack of SB transposase immunoreactivity in tissues from a wild type mouse (inset). Before incubation with primary antibody tissues were subjected to heat-induced epitope retrieval using R&D Systems basic antigen retrieval solution (Catalog # CTS013). Staining was done using R&D Systems anti-mouse HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS002). See IHC Enzymatic Protocol 1.0. Tissues were provided by Dr. Lara Collier at the University of Minnesota.

This antibody specifically recognizes Sleeping Beauty Transposase. This antibody has not yet been tested in other species. This antibody can be used for immunohistochemistry and Western blot.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multicolor fluorescence microscopy.