Wnt-9a in Human Breast Cancer Tissue.
Wnt-9a was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Wnt-9a Monoclonal Antibody (Catalog # MAB31571) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
This antibody specifically recognizes human Wnt-9a. In ELISA experiments, this antibody shows no cross-reactivity with recombinant human Wnt-2, -7a, or -7b
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.