Myeloperoxidase (MPO) was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Human/Mouse MPO Monoclonal Antibody (Catalog # MAB3174) at 8 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Myeloperoxidase (MPO) was detected in immersion fixed paraffin-embedded sections of human spleen using Human/Mouse MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Myeloperoxidase (MPO) was detected in immersion fixed paraffin-embedded sections of human spleen using Human/Mouse MPO Monoclonal Antibody (Catalog # MAB3174) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

These antibodies specifically recognize human/mouse Myeloperoxidase. Reactivity with Myeloperoxidase from other species has not been determined.