Plexin C1 was detected in immersion fixed paraffin-embedded sections of mouse embryo using Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to neurites in developing midbrain. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

This antibody specifically recognizes mouse Plexin C1. In ELISA and Western blot experiments, this antibody shows less than 1% cross-reactivity with recombinant mouse Plexin A1, A2, A3, and recombinant human Plexin B1, B2 and D1.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.