PTEN-induced Putative Kinase 1 (PINK1) was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human PINK1 Monoclonal Antibody (Catalog # MAB4357) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

This antibody specifically recognizes human PINK1 by ELISA and immunohistochemistry. Reactivity of this antibody with PINK1 from other species has not been determined.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.