CD19 in Rat Spleen.
CD19 was detected in perfusion fixed frozen sections of rat spleen using Mouse Anti-Rat CD19 Monoclonal Antibody (Catalog # MAB7489) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to B-cells inside the germinal layers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.