Technical Information

Technical Information

IHC/ICC Protocols

R&D Systems offers a range of high quality products for IHC/ICC experiments. Our primary antibodies are manufactured under controlled conditions to ensure lot-to-lot consistency, high specificity, and outstanding performance. The NorthernLights™ range of fluorescent secondary antibodies and streptavidin conjugates are bright, resistant to photobleaching and are ideal for multi-color fluorescence microscopy. In addition, we offer complete Cell & Tissue Staining Kits which contain all the materials required for visualization based on enzymatic conversion of the chromogenic substrates DAB and AEC.

The most challenging aspect of the IHC/ICC technique is determining the precise experimental conditions required to generate a strong and specific signal for each antigen of interest. To facilitate successful IHC/ICC experiments, R&D Systems provides specific protocols for staining of cells and tissues. For additional support, please refer to our troubleshooting guide, IHC/ICC image gallery, and other related resources.

IHC/ICC Protocols

Further IHC/ICC Support