Proteome Profiler Arrays
Many Proteome Profiler arrays are amenable to near-infrared (NIR) fluorescence detection using the LI-COR Odyssey®
Infrared Imaging System. To achieve this adaption, the HRP-conjugated Streptavidin provided in the kit is replaced with IRDye®
800CW Streptavidin, and the arrays are scanned using a LI-COR Odyssey Infrared Imaging System. This protocol is not applicable to all Proteome Profiler array kits. It is not applicable to arrays that use an HRP-conjugated detection antibody instead of Streptavidin-HRP, and is not recommended for arrays with phospho-specific antibodies due to increased signal noise. Representative data using LI-COR detection are shown for the Proteome Profiler array kits that have been tested. Proteome Profiler array kits that have not been tested may or may not be applicable to the LI-COR Odyssey®
Infrared Imaging System, or may require optimization by the customer.