Luminex® xMAP™ technology is based on the ability to measure multiple analytes in a single, sample volume. The key to this technology is the use of microparticle beads. These polystyrene beads are dyed with different ratios of two fluorochromes such that 100 unique spectral addresses are created. Each address is assigned a bead region number. When measuring multiple analytes in the same sample, each analyte needs to use a different bead region to differentiate it from the others. The chart below summarizes which bead regions are used for each of our Fluorokine MAP panel analytes.
| Bead Regions |
Human Panel A |
Human Panel B |
Human MMP Panel |
Human Obesity Panel |
Human Adhesion Molecule Panel |
Mouse Panel |
Rat Panel |
| 2 |
|
|
|
Adiponectin |
|
|
|
| 4 |
|
|
MMP-1 |
|
sP-Selectin/ CD62P |
|
TNF-alpha |
| 5 |
IL-1 alpha |
|
|
|
|
|
|
| 6 |
IL-1 beta |
|
|
|
|
IL-1 beta |
IL-1 alpha |
| 7 |
|
IL-12 p70 |
|
|
|
|
|
| 8 |
|
|
|
C-Reactive Protein |
|
|
|
| 9 |
IL-5 |
|
|
|
|
|
|
| 10 |
|
|
|
Serpin E1/ PAI-1 |
|
|
IL-6 |
| 11 |
GM-CSF |
|
|
Complement Factor D |
|
GM-CSF |
|
| 12 |
|
|
|
Resistin |
|
|
|
| 13 |
|
|
MMP-2 |
|
|
|
|
| 16 |
IL-1ra |
|
|
|
|
|
|
| 17 |
IL-2 |
|
|
|
|
IL-2 |
GM-CSF |
| 18 |
|
CD40L |
|
|
sICAM-1/ CD54 |
|
IL-18 |
| 19 |
|
|
|
|
|
|
IL-4 |
| 20 |
IL-17 |
|
|
|
sVCAM-1/ CD106 |
|
|
| 21 |
IL-4 |
|
|
|
|
IL-4 |
IL-1 beta |
| 22 |
|
CCL11/ Eotaxin |
|
|
|
|
|
| 26 |
|
|
MMP-3 |
|
|
CXCL1/KC |
IL-10 |
| 30 |
|
|
MMP-7 |
|
|
CCL2/ MCP-1/JE |
|
| 31 |
|
Leptin |
|
Leptin |
|
|
|
| 32 |
IL-6 |
|
|
IL-6 |
|
IL-6 |
|
| 33 |
|
|
|
|
|
|
TIMP-1 |
| 34 |
TPO |
|
|
|
|
|
L-Selectin/ CD62L |
| 35 |
|
IL-13 |
|
|
|
|
|
| 36 |
CXCL8/ IL-8 |
|
|
|
|
IL-13 |
|
| 37 |
CXCL5/ ENA-78 |
|
|
|
|
|
|
| 39 |
|
CXCL10/ IP-10 |
|
|
sE-Selectin/ CD62E |
IL-17 |
|
| 43 |
|
|
MMP-8 |
|
|
VEGF |
|
| 47 |
|
|
MMP-9 |
|
|
CXCL2/MIP-2 |
|
| 50 |
IL-10 |
|
|
IL-10 |
|
IL-10 |
|
| 51 |
|
EGF |
|
|
|
|
|
| 52 |
VEGF |
|
|
|
|
|
|
| 53 |
|
HGF |
|
|
|
|
CXCL3/CINC-2 alpha/beta |
| 54 |
FGF basic |
|
|
|
|
IL-5 |
CXCL2/CINC-3 |
| 58 |
G-CSF |
|
|
|
|
|
VEGF |
| 59 |
CCL3/ MIP-1 alpha |
|
|
|
|
|
|
| 60 |
|
CXCL11/ I-TAC |
|
|
|
|
|
| 61 |
|
|
|
|
|
|
IL-2 |
| 66 |
|
|
MMP-13 |
|
|
IL-12 p70 |
|
| 71 |
GM-CSF |
|
|
|
|
GM-CSF |
|
| 74 |
CCL4/ MIP-1 beta |
|
|
|
|
|
IL-13 |
| 75 |
IFN-gamma |
|
|
|
|
IFN-gamma |
ICAM-1/ CD54 |
| 77 |
TNF-alpha |
|
|
TNF-alpha |
|
|
|
| 78 |
CCL2/ MCP-1 |
|
|
CCL2/ MCP-1 |
|
|
|
| 79 |
|
|
|
|
|
TNF-alpha |
|
| 80 |
CCL5/ RANTES |
|
|
|
|
|
IFN-gamma |
Luminex is a registered trademark of Luminex Corporation.
Luminex xMAP is a trademark of Luminex Corporation.