• ELISpot Development Modules
• ELISpot Kits
• Other ELISAs/Assays

Introduction

R&D Systems' ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Complete ELISpot kits are ready-to-run and require no assay development or refinement. ELISpot Development Modules contain matched antibodies for the development of a working assay. Additional supplies are required and assay development expertise is recommended.

Features

• Detect and quantitate individual cells secreting specific analytes
• High sensitivity-can measure responses with frequencies well below 1/100,000 cells
• No in vitro expansion of cells required
• High-throughput-ELISpot assays use only a small volume of primary cells

Complete Kit Components

• Coated PVDF-backed 96-well Microplate
• Detection Antibody
• Streptavidin-AP
• BCIP/NBT
• Positive Control
• Dilution Buffers
• Wash Buffer

ELISpot Development Modules

ELISpot development modules are an alternative to ELISpot kits. A basic understanding of ELISpot assay development is required for the successful use of these reagents. Each investigator should optimize the experimental conditions, such as cell type, cell stimulation conditions and cell dilutions for each assay. The type of enzyme and substrate, as well as the concentrations of the capture and detection antibodies can give varied results.

The analyte-specific ELISpot Development Modules and the ELISpot Blue Color Module contain the necessary components for analyte detection and visualization, respectively. These modules can be used together but are sold separately. Each module contains enough reagents for at least 5 96-well microplates.

• Capture Antibody Concentrate
• Detection Antibody Concentrate

• Streptavidin-AP
• BCIP/NBT

References

1. Absence Of The Complement Anaphylatoxin C3a Receptor Suppresses Th2 Effector Functions In A Murine Model Of Pulmonary Allergy.
   Drouin, SM. et al. (2002) J Immunol, 169:5926-33
   Sample Type: Whole Lung Cells
   Catalog #: SEL404, SEL485
2. Encapsulated Plasmid Dna Treatment For Human Papillomavirus 16-associated Anal Dysplasia: A Phase I Study Of Zyc101.
   Klencke, B. et al. (2002) Clin Cancer Res, 8:1028-37
   Sample Type: PBMCs after pDNA vaccine
   Catalog #: EL285
3. Frequency Of Measles Virus-specific Cd4(+) And Cd8(+) T Cells In Subjects Seronegative Or Highly Seropositive For Measles Vaccine.
   Ovsyannikova, IG. et al. (2003) Clin Diagn Lab Immunol, 10:411-6
   Sample Type: T-Cells
   Catalog #: SEL285, SEL204
4. Prevention Of Graft-versus-host Disease While Preserving Graft-versus-leukemia Effect After Selective Depletion Of Host-reactive T Cells By Photodynamic Cell Purging Process.
   Chen, BJ. et al. (2002) Blood, 99:3083-8
   Sample Type: BALB/c, C3H/HeJ and irradiated (100 Gy) BCL1 cells
   Catalog #: EL485
5. Production Of Interleukin-13 By Human Dendritic Cells After Stimulation With Protein Allergens Is A Key Factor For Induction Of T Helper 2 Cytokines And Is Associated With Activation Of Signal Transducer And Activator Of Transcription-6.
   Bellinghausen, I. et al. (2003) Immunology, 108:167-76
   Sample Type: Dendritic Cells
   Catalog #: EL213, SEL213