Base Kit: LMP000 Human Fluorokine MAP Base Kit, MMP Panel
| Analyte |
Catalog Number |
Cell Culture |
Serum |
Heparin Plasma |
Sensitivity (pg/mL) |
High Standard Value* (pg/mL) |
Cross Reactivity/Interference |
| MMP-1 |
LMP901 |
X |
X |
X |
6.3 |
7400 |
hMMP-7: 0.9% x-react |
| MMP-2 |
LMP902 |
X |
X |
X |
38.9 |
58,000 |
mMMP-2: 11.9% x-react
hTIMP-2 -1.6% interference |
| MMP-3 |
LMP513 |
X |
X |
X |
2.6 |
10,900 |
hMMP-10: 3.0% x-react
mMMP-3: 0.7% x-react |
| MMP-7 |
LMP907 |
X |
X |
X |
29.5 |
72,000 |
|
| MMP-8 |
LMP908 |
X |
X |
X |
12.5 |
63,000 |
hMMP-10: 1.0% x-react
hTIMP-2: 1.3% x-react
mMMP-3: 0.6% x-react
mMMP-9: 0.8% x-react |
| MMP-9 |
LMP911 |
X |
X |
X |
11.0 |
18,000 |
hTIMP-1: 1.4% interference |
| MMP-12 |
LMP919 |
X |
X |
X |
1.9 |
9500 |
hTIMP-2: 0.7% interference
mMMP-2: 1.8% interference
mTIMP-1: 2.9% interference
rTIMP-1: 0.6% interference |
| MMP-13 |
LMP511 |
X |
X |
No |
28.6 |
60,000 |
|
All measure pro-, mature, and TIMP-1 complexed forms.
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. |
Sample Preparation
Total prepared sample volume needed per well is 50 microliters.
Serum and plasma samples require a 10-fold dilution. For testing samples in duplicate, a suggested 10-fold dilution is 15 microliters of sample + 135 microliters of Calibrator Diluent RD5-37. When assaying MMP-9, serum/plasma samples must be further diluted 10-fold to a final 100-fold dilution. A suggested 100-fold dilution is 15 microliters of the 10-fold diluted sample + 135 microliters of Calibrator Diluent RD5-37. The minimum starting serum/plasma volume recommended for sample dilution is 10 microliters. While 50 microliters of prepared (diluted) sample is added to each well, the actual serum/plasma volume in each well will be less than 10 microliters. Pipetting less than 10 microliters of serum/plasma is not recommended due to potential pipetting inaccuracy. Samples may be further diluted if necessary to bring sample data within the standard curve range.
Cell culture supernate samples require a 5-fold dilution. For testing samples in duplicate, a suggested 5-fold dilution is 25 microliters of sample + 100 microliters of Calibrator Diluent RD5-37. Samples may be further diluted if necessary to bring sample data within the standard curve range.