Fluorokine® MAP Multiplex Kits| Fluorokine MAP Multiplex Human Cytokine Panel A

• Ordering Information
• Product Insert PDF
• Assays for analytes represented in the Human Cytokine Panel A

Base Kit: LUH000 Human Fluorokine MAP Base Kit, Panel A

Analyte	Catalog Number	Cell Culture	Serum	EDTA Plasma	Heparin Plasma	Sensitivity (pg/mL)	High Standard Value* (pg/mL)	Microparticle Region
CCL2/MCP-1	LUH279	X	X	X	X	2.54	2000	78
CCL3/MIP-1 alpha	LUH270	X	X	X	X	8.45	6400	59
CCL4/MIP-1 beta	LUH271	X	X	X	X	0.72	4200	74
CCL5/RANTES	LUH278	X	X	X	X	1.91	1700	80
CXCL8/IL-8	LUH208	X	X	X	X	1.97	2500	36
CXCL5/ENA-78	LUH254	X	X	X	X	4.14	4000	37
FGF basic	LUH233	X	X	X		4.91	2200	54
G-CSF	LUH214	X	X	X	X	1.48	2400	58
GM-CSF	LUH215C	X	X	X	X	1.98	1800	11
IFN-gamma	LUH285	X	X	X	X	1.27	1900	75
IL-1 alpha/IL-1F1	LUH200B	X	X	X	X	1.15	3770	05
IL-1ra/IL-1F3	LUH280	X	X	X	X	5.23	5000	16
IL-1 beta/IL-1F2	LUH201	X	X	X	X	0.57	1920	06
IL-2	LUH202	X	X	X	X	2.23	2320	17
IL-4	LUH204	X	X	X	X	4.46	2200	21
IL-5	LUH205	X	X	X	X	0.71	1300	09
IL-6	LUH206	X	X	X	X	1.11	2400	32
IL-10	LUH217	X	X	X	X	0.30	2320	50
IL-17	LUH317	X	X	X	X	1.10	2100	20
TNF-alpha	LUH210	X	X	X	X	1.50	3900	77
Tpo	LUH288	X	X	X	X	9.94	10,000	34
VEGF	LUH293	X	X	X	X	1.84	2400	52
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Sample Preparation

Total prepared sample volume needed per well is 50 microliters.

Serum and plasma samples require a 4-fold dilution. For testing samples in duplicate, a suggested 4-fold dilution is 30 microliters of sample + 90 microliters of Calibrator Diluent RD6-40.

Cell culture supernates can be run undiluted with no matrix effects or interference. Maximum sample requirement per well is 50 microliters. Samples may be further diluted if necessary to bring sample data within the standard curve range.