Base Kit: LUB000 Human Fluorokine MAP Base Kit, Panel B
| Analyte |
Catalog Number |
Cell Culture |
Serum |
EDTA Plasma |
Heparin Plasma |
Sensitivity (pg/mL) |
High Standard Value* (pg/mL) |
Microparticle Region |
| CCL11/Eotaxin |
LUB320 |
X |
X |
X |
X |
8.2 |
12,000 |
22 |
| CD40L/TNFSF5 |
LUB201 |
X |
X |
X |
X |
23.4 |
71,600 |
18 |
| CXCL10/IP-10 |
LUB266 |
X |
|
|
X |
0.92 |
2300 |
39 |
| CXCL11/I-TAC |
LUB672 |
X |
X |
X |
X |
22.5 |
17,000 |
60 |
| EGF |
LUB236 |
X |
X |
X |
X |
1.58 |
2700 |
51 |
| HGF |
LUB294 |
X |
X |
X |
X |
4.1 |
23,000 |
53 |
| IL-12 p70 |
LUB219 |
X |
X |
X |
X |
14.9 |
34,000 |
07 |
| IL-13 |
LUB213 |
X |
X |
X |
X |
15.6 |
28,000 |
35 |
| Leptin (OB) |
LUB398 |
X |
X |
X |
X |
20.2 |
59,000 |
31 |
| *A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. |
Sample Preparation
Total prepared sample volume needed per well is 50 microliters.
Serum and plasma samples require a 4-fold dilution. For testing samples in duplicate, a suggested 4-fold dilution is 30 microliters of sample + 90 microliters of Calibrator Diluent RD6-43.
When assaying IL-12 p70, cell culture supernate samples require a 2-fold dilution. For testing samples in duplicate, a suggested 2-fold dilution is 75 microliters of sample + 75 microliters of Calibrator Diluent RD5-3. When assaying CXCL10/IP-10, cell culture samples require a 15-fold dilution. For testing samples in duplicate, a suggested 15-fold dilution is 10 microliters of sample + 140 microliters of Calibrator Diluent RD5-3. If IL-12 p70 and CXCL10/IP-10 are not being assayed, cell culture supernates can be run undiluted with no matrix effects or interference. Maximum sample requirement per well is 50 microliters. Samples may be further diluted if necessary to bring sample data within the standard curve range.