Introduction
The Human Phospho-Mitogen-activated Protein Kinase (MAPK) Antibody Array (Catalog # ARY002) is an economical tool used to simultaneously detect the relative phosphorylation of 19 kinases without performing numerous immunoprecipitation and/or Western blots. Kinases are captured by 21 different antibodies spotted in duplicate on a nitrocellulose membrane. Levels of phosphorylated protein are then assessed using phospho-specific antibodies and chemiluminescent detection.
| Simultaneously detect the relative phosphorylation of these kinases in a single sample. |
| ERK1 |
p38 alpha |
Akt1 |
| ERK2 |
p38 beta |
Akt2 |
| JNK1 |
p38 gamma |
Akt3 |
| JNK2 |
p38 delta |
Akt pan |
| JNK3 |
RSK1 |
GSK-3 alpha/beta |
| JNK pan |
RSK2 |
GSK-3 beta |
| MSK2 |
p70 S6K |
HSP27 |
Product Features
- SPECIFIC - Carefully selected panel of 21 capture antibodies gives low cross-reactivity
- RAPID - 5 hour assay time
- EFFICIENT - Tests 19 different kinases simultaneously with a small sample size
- SENSITIVE - Comparable to IP/Western Blot
Kit Contents
Each Human MAPK Antibody Array Kit contains most of the necessary components for a ready-to-run assay without the need for specialized equipment.*
- 4-Single Use Nitrocellulose Membranes spotted in duplicate with capture and control antibodies
- 4-Well Multi-dish
- Lysis Buffer 6
- Array Buffer 1 (for blocking and sample incubation)
- Array Buffer 2/3 (for dilution of the detection antibody)
- Wash Buffer Concentrate, 25X (for washing the arrays after lysate and detection antibody incubations)
- Anti-Phospho-Kinase Detection Antibody
- Streptavidin-HRP
- Transparency Overlay Template
- Detailed product insert with protocol
*Chemiluminescent detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the
product insert (PDF, 335K).