MagCellect

R&D Systems' MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic). By virtue of their small size (~150 nm diameter), MagCellect Ferrofluids behave like colloidal particles, easily remaining in solution and allowing for efficient diffusion kinetics in the binding reaction.

Complete Listing of MagCellect Kits
Enrich For

• CD3⁺ T Cells
• CD4⁺ or CD8⁺ T Cells
• Memory or Naïve CD4⁺ T Cells
• Naïve CD8⁺ T Cells
• B Cells
• CD4⁺CD25⁺ Regulatory T Cells
• CD14⁺
• TREM-1⁺
• Lineage Depletion

Species

• Human
• Mouse
• Rat

Kit Contents

• MagCellect Antibody Cocktail
• MagCellect Ferrofluid - conjugated to either goat anti-mouse IgG or streptavidin
• MagCellect 10X Buffer

MagCellect stand alone reagents

• MagCellect Goat Anti-mouse IgG Ferrofluid
• MagCellect Streptavidin Ferrofluid

MagCellect Magnet

To accommodate different processing capacities, this neodymium iron boron permanent magnet can hold six 12 x 75 mm (5 mL) or two 17 x 100 mm (15 mL) test tubes.

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Figure 1. Mouse splenocytes before (A) and after (B) separation of CD3+ T cells using R&D Systems' MagCellect CD3+ T Cell Isolation Kit (Catalog # MAGM201) Histograms reflect viable cells stained with CD3-FITC. Purity of all isolated cells for this experiment was 97.5%.
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Figure 2. Ficolled human PBMCs before (A) and after (B) separation of B cells using R&D Systems' MagCellect Human B Cell Isolation Kit (Catalog # MAGH103). Histograms reflect viable cells stained with CD19-FITC. Purity of all isolated cells for this experiment was 98%.