Fluorokine MAP Assay Principle for the Multiplex Kits
Fluorokine MAP Assay Principle for the Multiplex Kits

Slide 1

Analyte specific antibodies are pre-coated onto color-coded microparticles.

Slide 2

Analyte specific antibodies capture the analyte of interest.

Slide 3

Biotinylated antibodies specific to the analyte of interest are added and form an antibody-antigen sandwich.

Slide 4

Streptavidin-PE is added.
The microparticles are read using a Luminex® 100™ analyzer. One laser classifies the microparticle and determines the analyte that is being detected and the second laser determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of bound analyte.


Typical standard curves for IL-1β, IL-10, and IL-6 run simultaneously.
 

RELATED INFORMATION
  • MEROPS: The Peptidase Database
  • Multiplex Kits for the Luminex Platform
  • Technical Notes: Multi-Cytokine Profiling
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