R&D Systems now offers products for the development of user-defined microparticle-based multiplex immunoassays. The Goat Anti-Mouse IgG Coated Microparticle Packs allow for fast and efficient coupling of mouse antibodies to microparticle beads. The Multiplex Assay Accessory Pack, in conjunction with the Microparticle Packs, provides reagents sufficient for multiplexing up to ten analytes on a single plate.
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| Figure 1: Standard curves were generated using R&D Systems'
Matched Antibody Pairs Human IL-2 (Catalog # BAF202, MAB602,
and 202-IL) and Human Leptin (Catalog # 398-LP,
BAM398,
and MAB398)
in conjunction with the Fluorokine MAP Multiplex Development Base Kit.
Although this is typical data for an optimized assay, results may vary. |
Development Base Kit
- Microparticle Diluent
- Biotin Antibody Diluent 2
- Wash Buffer Concentrate
- Streptavidin-Conjugated Phycoerythrin
- Microplate
- Plate Covers
*Reagents sufficient for 1 plate
Microparticle Pack Contents
- Goat Anti-Mouse IgG Coated Microparticles
- Mixing Bottle
*Reagents sufficient for 1 plate
Additional components required:
- Luminex® 100™ Analyzer with X-Y Platform
- Mouse monoclonal antibodies for analytes of interest
- Biotin-labeled antibodies for analytes of interest
- Analytes of interest for construction of standard curve
- Buffer for the preparation of standards and the dilution of samples.
- Suitable for use with R&D Systems Matched
Antibody Pairs
Limitations
- A basic understanding of immunoassay development is required.
- This is not a validated assay. If used with other validated multiplex panels, the entire panel must be optimized and performance parameters verified.
Immunoassay References
- Armstrong, A.S. et al. (2001) in The Immunoassay Handbook, Wild, D. ed., Nature Publishing Group, New York, NY.
- Achord, D. et al. (1991) in Principles and Practice of Immunoassay, Price, C.P. and D.S. Newman, eds., Stockton Press, New York, NY.
- Crowther, J.R. (2001) in The ELISA Handbook, Humana Press, Totowa, NJ.
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Luminex 100 is a trademark of Luminex Corporation