Introduction
R&D Systems' new MycoProbe® Mycoplasma Detection Kit (Catalog # CUL001B)
represents a major breakthrough for high-throughput, routine screening of cell
cultures for mycoplasma contamination. Mycoplasma contamination is one of the
most common and serious problems in culturing eukaryotic cells because it alters
the phenotypic characteristics of the cells and can negatively impact results.
Since mycoplasma is typically not visible and does not respond to antibiotics,
an alternative sensitive and reliable detection method is required. This assay
is capable of detecting the eight mycoplasma species known to cause 95% of
eukaryotic cell culture contamination.
| CFU* |
MycoProbe® Mycoplasma Detection Kit |
PCR |
| 15,000 |
+ |
+ |
| 1,500 |
+ |
+ |
| 150 |
+ |
+ |
| 15 |
+ |
+ |
Table 1. Comparison
of R&D Systems MycoProbe® Mycoplasma Detection
Assay (Catalog # CUL001B),
with a commercially available PCR-based mycoplasma detection kit. Serial
dilutions of M.arginini were produced in lysis buffer.
*CFU = Colony Forming Units per well or PCR reaction
|
Assay Features
Kit Contents
- Each MycoProbe® Mycoplasma Detection Kit contains all of the necessary components for a ready-to-run assay and requires no additional development.
- Cell Lysis Buffer
- Hybridization Plate
- Streptavidin Capture Plate
- Diluents
- Substrate
- Conjugate
- Pan-Specific Oligonucleotide Probes
- Synthetic DNA Oligonucleotide Positive Control
- Protocol Manual
Mycoplasmas Detected
- M. hyorhinis
- M. arginini
- M. fermentans
- M. orale
- M. pirum
- M. hominis
- M. salivarium
- A. laidlawii