Proteome Profiler™ Antibody Arrays

Measure more than 100 proteins in a single sample. No specialized equipment required.

What are Proteome Profiler™ Antibody Arrays?

Proteome Profiler Antibody Arrays are simple and economical multiplex assays for early-stage discovery research.

This semiquantitative antibody array allows for the measurement of more than 100 proteins in a single sample. It requires no specialized equipment and eliminates the need for multiple Western blot experiments. Antibody array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high quality capture antibodies. The arrays utilize chemiluminescence for detection data that can be quantified in the same manner as traditional Western blots.

Select antibody arrays are also suitable for use with the LI-COR^® and FluorChem^™ detection systems.

Ready for a more quantitative multiplex assay? Try R&D Systems^® Luminex^® Assays.

Proteome Profiler Target Analytes

Choose from a wide variety of target analytes, including adipokines, chemokines, cytokines, kinases and proteases.

Multiplex Extracellular Target Analytes	Multiplex Intracellular Target Analytes
Human, Mouse, and Rat Cytokine Antibody Array	Human and Mouse Phospho-RTK Antibody Array
Human, Mouse, and Rat XL Cytokine Antibody Array	Human Phospho-Kinase Antibody Array
Human XL Oncology Antibody Array	Human Cell Stress Antibody Array
Human, Mouse, and Rat Adipokine Antibody Array	Human and Mouse Apoptosis Array
Human and Mouse Angiogenesis Antibody Array	Human Pluripotent Stem Cell Antibody Array
Human and Mouse Chemokine Antibody Array	Human Phospho-Immunoreceptor Antibody Array
Human Protease Antibody Array	Human Ubiquitin Antibody Array
Human Protease Inhibitor Antibody Array	Human NFkB Pathway Antibody Array
Human Protease/Protease Inhibitor Antibody Array
Human Soluble Receptor Antibody Array, Non-Hematopoietic Panel

Antibody Array Principle

Capture antibodies are spotted in duplicate on the nitrocellulose membrane.
Spotted membrane arrays are incubated in a single lysate.
Target analytes are captured by the capture antibody.
Target analytes are detected using a biotinylated or horseradish peroxidase (HRP)-conjugated detection antibody.
Biotinylated detection antibodies are reacted with streptavidin HRP.
Membranes are incubated in a chemiluminescence reagent that produces a light signal proportional to the amount of antibody-target analyte complexes formed.
Data is visualized using standard western blotting methods such as film or digital cameras.

Proteome Profiler Antibody Arrays graphic assay principle

Quick Spots Image Analysis Software

R&D Systems^® now offers Quick Spots image analysis software designed specifically for your Proteome Profiler^™ Arrays.

No more laborious set-up of templates in other imaging software, Quick Spots contains preloaded templates for each Proteome Profiler^™ array. It knows where your results should be and provides the data in less time and with less work.

Download Quick Spots

Quick Spots software for antibody array data analysis.

Antibody Array Protocol Video

Watch this video to learn how Proteome Profiler Antibody Arrays are used to profile changes in Receptor Tyrosine Kinase Phosphorylation.

Webinar – Arrays in Cancer Research

Watch this video to learn how Dr. Xin Lu and his research team used Proteome Profiler Antibody Arrays to understand the role that immunosuppressive myeloid cells play in prostate cancer.