Product Category
Angiogenesis Assays
The Cultrex® Directed In Vivo Angiogenesis Assays (DIVAA™) are designed to assess angiogenesis in an in vivo system. The DIVAA kits provide implant grade silicone cylinders closed at one end, called angioreactors, that are implanted subcutaneously into the dorsal flank of mice.a Prior to implantation, the angioreactors are filled by the end user with PathClear® basement membrane extract (BME) premixed with or without angiogenesis modulating factors. With the onset of angiogenesis, vascular endothelial cells proceed to grow into the BME and form vessels in the angioreactor. The total number of endothelial cells contained within the angioreactor can then be determined by fluorometric detection using the FITC-conjugated Lectin supplied in the kit and a standard microplate reader. Compared with other commonly used angiogenesis assays, the DIVAA kits provide a standardized platform for reproducible and quantifiable assessment of angiogenesis in vivo.
Available DIVAA Kits
DIVAA Starter Kit – The Directed In Vivo Angiogenesis Assay Starter Kit (Catalog # 3450-048-SK) is designed to give new users practical experience using the angioreactors and is ideal for optimizing assay conditions. Each kit contains 48 angioreactors and enough growth factor to induce angiogenesis in all of the provided angioreactors. The DIVAA Starter Kit also supplies an autoclavable and reusable AngioRack™ to hold the angioreactors while they are being prepared for implantation.
DIVAA Activation Kit – The Directed In Vivo Angiogenesis Assay Activation Kit (Catalog #
3450-048-K) is designed for assessing angiogenesis activation under your experimental conditions. Each kit contains 48 angioreactors and enough growth factor for eight positive controls.
DIVAA Inhibition Kit – The Directed In Vivo Angiogenesis Assay Inhibition Kit (Catalog #
3450-048-IK) is designed for assessing angiogenesis inhibition. Each kit contains 48 angioreactors and enough growth factor to induce angiogenesis in all 48 experiments.
Features
- Utilizes a standardized platform for reproducible and quantifiable results
- Results seen in as few as 9 days post-implantation
- Up to four angioreactors can be implanted in each mouse, allowing for greater statistical power
- Uses a fraction of materials conserving BME and test compounds
- Averts assay errors caused by absorption of BME by the mouse
Kit Contents
- Angioreactors
- Growth Factor Reduced PathClear BMEb
- 10X Wash Buffer
- FGF-2c
- CellSperse™
- 200X FITC-Lectin
- 25X FITC-Lectin Diluent
- Heparin Solution
- AngioRackd
- FGF-2 (1.8 μg)/VEGF (600 ng)
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Evaluation of Angiogenesis Activation using DIVAA. DIVAA was used to assess angiogenesis in nude mice stimulated by either fibroblast growth factor-2 alone (FGF-2, 100 ng) or in combination with vascular endothelial growth factor (FGF-2, 37 ng; VEGF, 12.5 ng). Angiogenesis was measured as the amount of FITC-Lectin detected 9 days post-implantation. Data is shown as the amount of fluorescence detected in treated samples (FGF-2, FGF-2/VEGF) versus the negative control (Untreated). |
- aThe DIVAA system is validated for use in nude mice. Optimization is required if using other mouse strains.
- bBME has been tested and cleared of 31 different pathogens and viruses.
- cFGF-2 is provided for a positive control in only the starter and activation kits.
- dAngioRack is only part of the starter kit.
Cultrex and PathClear are registered trademarks of Trevigen, Inc.
DIVAA, AngioRack, and CellSperse are trademarks of Trevigen, Inc.