• Complete Listing of Cell Culture Media & Supplements

Culture Media and Supplements

Mouse Embryonic Fibroblast Conditioned Media (Catalog # AR005)

Serum-free media (80% Knockout™ D-MEM, 20% Knockout serum replacement, 1% MEM non-essential amino acids, 2 mM GlutaMAX™, 0.1 mM beta-mercaptoethanol, 4 ng/mL FGF basic) was conditioned by gamma-irradiated CF-1 fibroblasts at 37° C for 24 hours.

Human Feeder Cell Conditioned Media (Catalog # AR007)

Media prepared using serum-free media (GlobalStem ES-DMEM™, 20% KSR™, and 4 ng/mL FGF basic) conditioned by primary human feeder cells at 37° C for more than 24 hours.

A

B

SSEA-4 and Oct-3/4 Expression in Embryonic Stem Cells. Human embryonic stem cells were cultured with recombinant human FGF basic (Catalog # 233-FB) in the presence (A) or absence (B) of Mouse Embryonic Fibroblast (MEF)-conditioned medium (Catalog # AR005). SSEA-4 and Oct-3/4 were detected using anti-human SSEA-4 monoclonal antibody (red, Catalog # MAB1435) and anti-human Oct-3/4 polyclonal antibody (green, Catalog # AF1759). Cells were counterstained with DAPI (blue). Image courtesy of Dr. Frank Soldner of the National Institutes of Health.

StemXVivo™ Culture Media

StemXVivo media products are specifically designed for stem/progenitor cell culture. All the components included within these defined, basal, serum-free formulations have been selected, optimized, and validated for the specific cell type of interest.

N-2 Plus Media Supplement

A serum-free, chemically defined, concentrated media supplement formulated to provide optimal growth conditions for neural stem cell expansion (Catalog # AR003). N2 is composed of Bovine Insulin, Human Transferrin, Putrescine, Selenite, and Progesterone. The supplement is supplied as a 100X concentrate in water.

Resazurin

Resazurin can be utilized as a simple and quantitative method for measuring cell proliferation, viability, and cytotoxicity. It is a non-toxic, water soluble, redox-sensitive dye that changes from its blue/non-fluorescent state to a pink/highly fluorescent state upon reduction by viable cells. Fluorescence can be read using 544 nm excitation and 590 nm emission wavelengths or absorbance can be read using a spectrophotometer set at 570 nm.

• Label: Resazurin changes from blue/non-fluorescent to pink/highly fluorescent
• Testing Format: Microplate reader
• Sample Type: Cultured cells
• Size: 100 mL (Catalog # AR002)

Additional Reagents

Extracellular Matrix Molecules
R&D Systems offers a range of recombinant ECM molecules which can be applied to cell culture plates to promote cell adhesion.

Basement Membrane Extracts
Basement Membrane Extracts promote and maintain differentiated phenotypes of cells in culture.

Cultrex Poly-L-Lysine
A highly positively charged amino acid chain commonly used as a coating agent to promote cell adhesion in culture. (Catalog # 3438-100-01)

Polystyrene Culture Microplates
R&D Systems offers packs of high-binding, flat-bottom polystyrene 96-well microplates. Each microplate consists of twelve removable strips of 8 wells and are available in both clear (Catalog # DY990) and black (Catalog # DY991).