Cell & Tissue Staining Kits
R&D Systems’ Cell and Tissue Staining Kits are intended for localization of antigens in a broad range of histological and cytological specimens. These kits may be used with primary IgG antibodies manufactured by either R&D Systems or by other vendors. Briefly, detection is based on the formation of the Avidin-Biotin Complex (ABC) with primary antibodies that reacted with tissue antigens under study. Visualization is based on enzymatic conversion of chromogenic substrates 3,3’ Diaminobenzidine (DAB) or 3-amino-9-ethylcarbazole (AEC) into a colored precipitate (brown and red, respectively), by horseradish peroxidase (HRP) at the sites of antigen localization, which can then be viewed using bright-field microscopy.
The high detection sensitivity of R&D Systems’ staining kits is achieved by using high quality Secondary Biotinylated Antibodies and High Sensitivity Streptavidin conjugated to HRP (HSS-HRP). High Sensitivity Streptavidin is a chemical analog of Streptavidin that has little net positive charge at neutral or slightly alkaline pH and will interact only with biotin attached to secondary antibodies. HSS-HRP shows little or no non-specific binding to phospholipids, nucleic acids, and carbohydrate binding proteins.
- Ready to use
- High Detection Sensitivity
- Suitable for cryostat, paraffin-embedded, and free-floating tissue sections
- Suitable for cytospin preparations (i.e. lymphocytes, monocytes, transfected cells)
- Available for goat, mouse, rabbit, rat, or sheep IgG primary antibodies
- Blocking reagents
- Secondary biotinylated antibodies
- High Sensitivity Streptavidin-HRP Conjugate (HSS-HRP)
- DAB (brown) or AEC (red) chromogen and buffer
|Wnt-2 in Human Stomach Cancer.
Wnt-2 was detected in paraffin-embedded human stomach cancer sections using 10 µg/mL goat anti-human Wnt-2 affinity-purified polyclonal antibody (Catalog # AF3464). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval kit (Catalog # CTS013). Tissue was stained using R&D Systems anti-goat HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). See IHC Enzymatic Protocol 1.0.
|Calbindin D in Rat Brain.|
Calbindin D was detected in paraffin-embedded rat brainstem medulla. Staining is shown in the medial vestibular nucleus using 25 µg/mL mouse anti-human Calbindin D monoclonal antibody (Catalog # MAB3320). Tissue was stained using R&D Systems anti-mouse HRP-AEC Cell and Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue).