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Comparison of Cell-Based ELISA and Cell-Based Infrared ELISA

Assay Principle

Assay can be completed in one day or primary antibodies can be
incubated overnight to increase sensitivity.

Cell-Based ELISA

(Fluorometric Detection)

Same Day Protocol:7 hrs 45 mins
Overnight Protocol:Day 1 = 2 hrs 30 mins
Day 2 = 4 hrs

Cell-Based Infrared ELISA

(Near-Infrared Detection)

Same Day Protocol:6 hrs 30 mins
Overnight Protocol:Day 1 = 2 hrs
Day 2 = 2 hrs 45 mins
Seed cells in a 96-well plate. Stimulate cells. Fix, permeabilize, and block cells. Seed cells in a 96-well plate. Stimulate cells. Fix, permeabilize, and block cells.
Add primary antibody cocktail. Add primary antibody cocktail.
Add HRP-conjugated and AP-conjugated secondary antibody cocktail. Add IRDye® 800CW-conjugated* and IRDye® 680LT-conjugated* secondary antibody cocktail and measure near-infrared fluorescence.
Add fluorogenic substrates and measure fluorescence

*This product is covered by US 6,995,274; US 7,504,089; foreign equivalents and patents pending.