 |
| Figure 1. Expansion of rat cortical stem cells. For monolayer culture, cells were expanded using R&D
Systems Fibronectin-coated plates (Catalog # 1030-FN) and StemXVivo
Serum-Free NSC Base Media (Catalog # CCM002) supplemented with recombinant
human FGF basic (Catalog # 233-FB; 20 ng/mL ). For neurosphere culture,
cells were expanded using the StemXVivo Serum-Free NSC Base Media supplemented
with recombinant human FGF basic and EGF (Catalog # 236-EG;
20 ng/mL). |
StemXVivo™ Media for Stem Cell Culture
Researchers employing stem or progenitor cells in their studies
currently use a wide variety of media products to support their cultures.
These media are generally developed or validated for cells other than stem
cells, thus making them less desirable than cell type-specific media. To
address this need, R&D Systems offers two new media products
for stem/progenitor cell culture. All the components included within these
defined, basal, serum-free formulations have been selected, optimized, and
validated for primary stem cell culture. StemXVivo Serum-free NSC Base Media is formulated
for expansion and differentiation of human and rodent neural stem and progenitor
cells.
Additional Media and Cell Culture Reagents