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Figure 1. R&D Systems Human Cytokine Array, Panel A
( Catalog # ARY005) utilizes capture
antibodies spotted onto a nitrocellulose membrane to allow high-throughput
multi-analyte profiling of 36 cytokines, chemokines, and acute phase proteins
in a single sample. Peripheral blood mononuclear cells treated with PHA for
24 hours were mixed with a cocktail of biotinylated detection antibodies, and
then incubated with the Human Cytokine Array. The array was then incubated
with streptavidin-horseradish peroxidase followed by chemiluminescent detection.
After detection, the array data were quantitated to generate a protein profile
(histogram). The table shows the analytes detected and their location on the
membrane. |