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Sample Data: Human Cytokine Array, Panel A |
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Figure 1. R&D Systems Human Cytokine Array, Panel A
( Catalog # ARY005) utilizes capture
antibodies spotted onto a nitrocellulose membrane to allow high-throughput
multi-analyte profiling of 36 cytokines, chemokines, and acute phase proteins
in a single sample. Peripheral blood mononuclear cells treated with PHA for
24 hours were mixed with a cocktail of biotinylated detection antibodies, and
then incubated with the Human Cytokine Array. The array was then incubated
with streptavidin-horseradish peroxidase followed by chemiluminescent detection.
After detection, the array data were quantitated to generate a protein profile
(histogram). The table shows the analytes detected and their location on the
membrane. |
| Location |
Analyte |
Location |
Analyte |
| A1 |
PC |
C6 |
IL-17 |
| A2 |
C5a |
C7 |
IL-17E |
| A3 |
CD40 L |
C8 |
IL-23 |
| A4 |
G-CSF |
C9 |
IL-27 |
| A5 |
GM-CSF |
C10 |
Blank |
| A6 |
GRO-α |
D1 |
Blank |
| A7 |
I-309 |
D2 |
IL-32α |
| A8 |
ICAM-1 |
D3 |
IP-10 |
| A9 |
IFN-γ |
D4 |
I-TAC |
| A10 |
PC |
D5 |
MCP-1 |
| B1 |
Blank |
D6 |
MIF |
| B2 |
IL-1α |
D7 |
MIP-1α |
| B3 |
IL-1β |
D8 |
MIP-1β |
| B4 |
IL-1ra |
D9 |
Serpin E1 |
| B5 |
IL-2 |
D10 |
Blank |
| B6 |
IL-4 |
E1 |
PC |
| B7 |
IL-5 |
E2 |
RANTES |
| B8 |
IL-6 |
E3 |
SDF-1 |
| B9 |
IL-8 |
E4 |
TNF-α |
| B10 |
Blank |
E5 |
TREM-1 |
| C1 |
Blank |
E6 |
Blank |
| C2 |
IL-10 |
E7 |
Blank |
| C3 |
IL-12 p70 |
E8 |
Blank |
| C4 |
IL-13 |
E9 |
Blank |
| C5 |
IL-16 |
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