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Human Cytokine Array Kit, Panel A

Introduction

R&D Systems Human Cytokine Array, Panel A (Catalog # ARY005) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 36 different cytokines, chemokines, and acute phase proteins in a single sample. No specialized equipment is necessary.

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
C5a IL-4 IL-32 alpha
CD40 ligand IL-5 CXCL10/IP-10
G-CSF IL-6 CXCL11/I-TAC
GM-CSF IL-8 CCL2/MCP-1
CXCL1/GRO alpha IL-10 MIF
CCL1/I-309 IL-12 p70 CCL3/MIP-1 alpha
ICAM-1 IL-13 CCL4/MIP-1 beta
IFN-gamma IL-16 CCL5/RANTES
IL-1 alpha IL-17 CXCL12/SDF-1
IL-1 beta IL-17E Serpin E1/PAI-1
IL-1ra IL-23 TNF-alpha
IL-2 IL-27 TREM-1


General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Antibody Detection Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.