Introduction
| Simultaneously detect the levels of these cytokines, chemokines,
and acute phase proteins in a single sample. |
| C5a |
IL-4 |
IL-32 alpha |
| CD40 ligand |
IL-5 |
IP-10/CXCL10 |
| G-CSF |
IL-6 |
I-TAC/CXCL11 |
| GM-CSF |
IL-8 |
MCP-1/CCL2 |
| GROa/CXCL1 |
IL-10 |
MIF |
| I-309/CCL1 |
IL-12p70 |
MIP-1 alpha/CCL3 |
| ICAM-1 |
IL-13 |
MIP-1 beta/CCL3 |
| IFN-gamma |
IL-16 |
RANTES/CCL5 |
| IL-1 alpha |
IL-17 |
SDF-1/CXCL12 |
| IL-1 beta |
IL-17E |
Serpin-E1/PAI-1 |
| IL-1ra |
IL-23 |
TNF-alpha |
| IL-2 |
IL-27 |
TREM-1 |
R&D Systems Human Cytokine Array, Panel A (Catalog # ARY005) is a rapid, sensitive, and economical tool used to simultaneously
detect the relative levels of 36 different cytokines, chemokines, and acute
phase proteins in a single sample. No specialized equipment is necessary.
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose
membranes. Cell culture supernatant, serum, or plasma samples are diluted and
mixed with a cocktail of biotinylated detection antibodies. The sample/antibody
mixture is then incubated with the array. Any cytokine/detection antibody complex
present is bound by its cognate immobilized capture antibody on the membrane.
Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents*
are added and a signal is produced in proportion to the amount of cytokine
bound.
Kit Contents
- 4 Array Membranes
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Transparency Overlay Template
- Detailed Protocol
*Chemiluminescence detection substrate not included. For a complete list
of the kit contents and necessary materials, please see the Materials Provided/Materials
Required sections of the product insert ( PDF 116 KB).