Product Category
Human Cytokine Array Kit, Panel A
Introduction
R&D Systems Human Cytokine Array, Panel A (Catalog # ARY005) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 36 different cytokines, chemokines, and acute phase proteins in a single sample. No specialized equipment is necessary.
| Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. |
| C5a |
IL-4 |
IL-32 alpha |
| CD40 ligand |
IL-5 |
CXCL10/IP-10 |
| G-CSF |
IL-6 |
CXCL11/I-TAC |
| GM-CSF |
IL-8 |
CCL2/MCP-1 |
| CXCL1/GRO alpha |
IL-10 |
MIF |
| CCL1/I-309 |
IL-12 p70 |
CCL3/MIP-1 alpha |
| ICAM-1 |
IL-13 |
CCL4/MIP-1 beta |
| IFN-gamma |
IL-16 |
CCL5/RANTES |
| IL-1 alpha |
IL-17 |
CXCL12/SDF-1 |
| IL-1 beta |
IL-17E |
Serpin E1/PAI-1 |
| IL-1ra |
IL-23 |
TNF-alpha |
| IL-2 |
IL-27 |
TREM-1 |
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents* are added and a signal is produced in proportion to the amount of cytokine bound.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Transparency Overlay Template
- Detailed Protocol
*Chemiluminescence detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert (PDF 116 KB).