The human genome is exposed to potentially deleterious genotoxic events during every cell division cycle. This endogenous source of DNA damage results from cellular metabolism or routine errors in DNA replication and recombination. In addition, cellular and organismal exposure to exogenous genotoxic agents such as ionizing radiation, ultraviolet light, oxidative stress, and chemical mutagens, may lead to a variety of nucleotide modifications and DNA strand breaks. Several assays are available to assess the mechanisms underlying DNA damage and repair.
R&D Systems colorimetric, ethidium bromide, luminescent, and isotope-based kits for detecting internucleosomal cleavage of DNA.
A colorimetric assay to detect Poly (ADP-ribose) Polymerase (PARP), an abundant enzyme that is activated upon DNA damage.
A simple and effective method for evaluating DNA damage in single cells.
Fragment Length Analysis using Repair Enzymes (FLARE) Assay Kits screen for DNA damage in single cells using specific DNA cleavage enzymes.
DNA Damage and Repair Enzymes can be used in vitro to detect specific forms of DNA damage.
Substrates for DNA Damage and Repair Enzymes serve as positive controls for DNA repair assays.
FLARE and CometAssay are trademarks of Trevigen, Inc.